IgG subclass-dependent pulmonary antigen retention during acute IgG-dependent systemic anaphylaxis in mice

Biliana Todorova (1) , Ophélie Godon (1) , Eva Conde (1) , Caitlin Gillis (1) , Bruno Iannascoli (1) , Odile Richard Le Goff (1) , Daniel Fiole (2, 3) , Lubka Roumenina (4) , Jeanette Leusen (5) , Andrew Murphy (6) , Lynn Macdonald (6) , Laurent Lionel Reber (1, 7) , Friederike Jönsson (1) , Pierre Bruhns (1)

1 Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology
2 Unité de Biothérapies anti-Infectieuses et Immunité [Brétigny-sur-Orge]
3 Histopathologie humaine et Modèles animaux
4 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers
5 University Medical Center [Utrecht]
6 Regeneron Pharmaceuticals [Tarrytown, NY]
7 Infinity - Institut Toulousain des Maladies Infectieuses et Inflammatoires

Mouse models of active systemic anaphylaxis rely predominantly on IgG Abs forming IgG–allergen immune complexes that induce IgG receptor–expressing neutrophils and monocytes/macrophages to release potent mediators, leading to systemic effects. Whether anaphylaxis initiates locally or systemically remains unknown. In this study, we aimed at identifying the anatomical location of IgG–allergen immune complexes during anaphylaxis. Active systemic anaphylaxis was induced following immunization with BSA and i.v. challenge with fluorescently labeled BSA. Ag retention across different organs was examined using whole-body fluorescence imaging, comparing immunized and naive animals. Various mouse models and in vivo deletion strategies were employed to determine the contribution of IgG receptors, complement component C1q, myeloid cell types, and anaphylaxis mediators. We found that following challenge, Ag diffused systemically, but specifically accumulated in the lungs of mice sensitized to that Ag, where it formed large Ab-dependent aggregates in the vasculature. Ag retention in the lungs did not rely on IgG receptors, C1q, neutrophils, or macrophages. IgG2a-mediated, but neither IgG1- nor IgG2b-mediated, passive systemic anaphylaxis led to Ag retention in the lung. Neutrophils and monocytes significantly accumulated in the lungs after challenge and captured high amounts of Ag, which led to downmodulation of surface IgG receptors and triggered their activation. Thus, within minutes of systemic injection in sensitized mice, Ag formed aggregates in the lung and liver vasculature, but accumulated specifically and dose-dependently in the lung. Neutrophils and monocytes recruited to the lung captured Ag and became activated. However, Ag aggregation in the lung vasculature was not necessary for anaphylaxis induction.

Mots clés: Allergens; Anaphylaxis; Animals; Antigen-Antibody Complex; Complement C1q

Domaines: Sciences du Vivant [q-bio]

Biliana Todorova, Ophélie Godon, Eva Conde, Caitlin Gillis, Bruno Iannascoli, et al.. IgG subclass-dependent pulmonary antigen retention during acute IgG-dependent systemic anaphylaxis in mice. Journal of Immunology, 2022, 209 (7), pp.1243-1251. ⟨10.4049/jimmunol.2200234⟩. ⟨pasteur-04222570⟩