Failure of multiplex meningitis/encephalitis (ME) NAT during cryptococcal meningitis in solid organ recipients

Benoit Pilmis (1, 2, 3) , Marie‐elizabeth Bougnoux (4) , Romain Guery (3) , Yaye Senghor (1) , Alban Le Monnier (1) , Fanny Lanternier (3, 5, 6) , Stephane Bretagne (7, 6, 5) , Alexandre Alanio (7, 6, 5) , Olivier Lortholary (3, 5, 6)

1 hpsj - Groupe Hospitalier Paris Saint-Joseph
2 UBaPS - Bactéries, Pathogènes et Santé
3 Centre d'infectiologie Necker-Pasteur [CHU Necker]
4 Hôpital Necker - Enfants Malades [AP-HP]
5 Mycologie moléculaire - Molecular Mycology
6 CNRMA - Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals
7 Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris]

Cryptococcal meningitis is a severe cause of central nervous system infections among immunocompromised solid organ transplant (SOT) patients. While new diagnostic methods as multiplex meningitis/encephalitis (ME) NAT (nucleic acid test) are increasingly used as a first‐line tool in hospital practice, data in HIV‐negative patients including SOT remain scarce. We report here false‐negative results of multiplex NAT among SOT patients with proven cryptococcal meningitis.

Mots clés: Cryptococcus sp.; meningitis/meningoencephalitis; multiplex PCR

Domaines: Santé; Maladies infectieuses; Bactériologie; Virologie; Santé publique et épidémiologie

Benoit Pilmis, Marie‐elizabeth Bougnoux, Romain Guery, Yaye Senghor, Alban Le Monnier, et al.. Failure of multiplex meningitis/encephalitis (ME) NAT during cryptococcal meningitis in solid organ recipients. Transplant Infectious Disease, 2020, 22 (4), ⟨10.1111/tid.13263⟩. ⟨hal-04316650⟩