Assessment of Mechanical Properties of Adherent Living Cells by Bead Micromanipulation: Comparison of Magnetic Twisting Cytometry vs Optical Tweezers - Archive ouverte HAL Access content directly
Journal Articles Journal of Biomechanical Engineering Year : 2002

Assessment of Mechanical Properties of Adherent Living Cells by Bead Micromanipulation: Comparison of Magnetic Twisting Cytometry vs Optical Tweezers

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Abstract

We compare the measurements of viscoelastic properties of adherent alveolar epithelial cells by two micromanipulation techniques: (i) magnetic twisting cytometry and (ii) optical tweezers, using microbeads of same size and similarly attached to F-actin. The values of equivalent Young modulus E, derived from linear viscoelasticity theory, become consistent when the degree of bead immersion in the cell is taken into account. E-values are smaller in (i) than in (ii): ∼34–58 Pa vs ∼29–258 Pa, probably because higher stress in (i) reinforces nonlinearity and cellular plasticity. Otherwise, similar relaxation time constants, around 2 s, suggest similar dissipative mechanisms.
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Dates and versions

hal-03876204 , version 1 (28-11-2022)

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Attribution - CC BY 4.0

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Cite

Valérie Laurent, Sylvie Hénon, Emmanuelle Planus, Redouane Fodil, Martial Balland, et al.. Assessment of Mechanical Properties of Adherent Living Cells by Bead Micromanipulation: Comparison of Magnetic Twisting Cytometry vs Optical Tweezers. Journal of Biomechanical Engineering, 2002, 124 (4), pp.408-421. ⟨10.1115/1.1485285⟩. ⟨hal-03876204⟩
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