The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium

Asma Boukhalfa (1, 2) , Federica Roccio (1) , Nicolas Dupont (1) , Patrice Codogno (1) , Etienne Morel (1)

1 INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades
2 Tufts Medical Center, Tufts University School of Medicine

The primary cilium (PC) regulates signalization linked to external stress sensing. Previous works established a functional interplay between the PC and the autophagic machinery. When ciliogenesis is promoted by serum deprivation, the autophagy protein ATG16L1 and the ciliary protein IFT20 are co-transported to the PC. Here, we demonstrate that IFT20 and ATG16L1 are part of the same complex requiring the WD40 domain of ATG16L1 and a Y-E-F-I motif in IFT20. We show that ATG16L1-deficient cells exhibit aberrant ciliary structures, which accumulate PI4,5P2, whereas PI4P, a lipid normally concentrated in the PC, is absent. Finally, we demonstrate that INPP5E, a phosphoinositide-associated phosphatase responsible for PI4P generation, interacts with ATG16L1 and that a perturbation of the ATG16L1/IFT20 complex alters its trafficking to the PC. Altogether, our results reveal a function of ATG16L1 in ciliary lipid and protein trafficking, thus directly contributing to proper PC dynamics and functions.

Domains: Life Sciences [q-bio]

Asma Boukhalfa, Federica Roccio, Nicolas Dupont, Patrice Codogno, Etienne Morel. The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium. Cell Reports, 2021, 35 (4), pp.109045. ⟨10.1016/j.celrep.2021.109045⟩. ⟨hal-03246943⟩