Identification of an IL-7-Dependent Pre-T Committed Population in the Spleen - Archive ouverte HAL Access content directly
Journal Articles Journal of Immunology Year : 2007

Identification of an IL-7-Dependent Pre-T Committed Population in the Spleen

(1) , , (2, 1) , (1) , , , (3) , (1)
1
2
3

Abstract

Several extrathymic T cell progenitors have been described but their various contributions to the T cell lineage puzzle are unclear. In this study, we provide evidence for a splenic Lin−Thy1.2+ T cell-committed population, rare in B6 mice, abundant in TCRα−/−, CD3ε−/−, and nude mice, and absent in IL-7- and Rag-2-deficient mice. Neither B nor myeloid cells are generated in vivo and in vitro. The incidence of these pre-T cells is under the control of thymus and/or mature T cells, as revealed by graft experiments. Indeed, IL-7 consumption by mature T cells inhibits the growth of these pre-T cells. Moreover, the nude spleen contains an additional Lin−Thy1.2+CD25+ subset which is detected in B6 mice only after thymectomy. We establish that the full pre-T cell potential and proliferation capacity are only present in the c-kitlow fraction of progenitors. We also show that most CCR9+ progenitors are retained in the spleen of nude mice, but present in the blood of B6 mice. Thus, our data describe a new T cell lineage restricted subset that accumulates in the spleen before migration to the thymus.

Dates and versions

hal-01996455 , version 1 (28-01-2019)

Identifiers

Cite

L. Gautreau, L. Arcangeli, V. Pasqualetto, A.-M. Joret, C. Garcia-Cordier, et al.. Identification of an IL-7-Dependent Pre-T Committed Population in the Spleen. Journal of Immunology, 2007, 179 (5), pp.2925-2935. ⟨10.4049/jimmunol.179.5.2925⟩. ⟨hal-01996455⟩

Collections

CNRS UNIV-PARIS
37 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More