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Journal Articles Blood Advances Year : 2018

Hematopoietic protease nexin-1 protects against lung injury by preventing thrombin signaling in mice

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Deborah François
  • Function : Author
Véronique Arocas
  • Function : Author
Laurence Venisse
  • Function : Author
Karen Aymonnier
  • Function : Author
Leila Idir
  • Function : Author
Raphael Martos
  • Function : Author
Salome Gazit
  • Function : Author
Ludovic Couty
  • Function : Author
Martine Jandrot-Perrus
  • Function : Author
Eric Camerer
Yacine Boulaftali
  • Function : Author
Marie-Christine Bouton
  • Function : Author

Abstract

Coagulation and fibrinolytic system deregulation has been implicated in the development of idiopathic pulmonary fibrosis, a devastating form of interstitial lung disease. We used intratracheal instillation of bleomycin to induce pulmonary fibrosis in mice and analyzed the role of serine protease inhibitor E2 (serpinE2)/protease nexin-1 (PN-1), a tissue serpin that exhibits anticoagulant and antifibrinolytic properties. PN-1 deficiency was associated, after bleomycin challenge, with a significant increase in mortality, as well as a marked increase in active thrombin in bronchoalveolar lavage fluids, an overexpression of extracellular matrix proteins, and an accumulation of inflammatory cells in the lungs. Bone marrow transplantation experiments showed that protective PN-1 was derived from hematopoietic cell compartment. A pharmacological strategy using the direct thrombin inhibitor argatroban reversed the deleterious effects of PN-1 deficiency. Concomitant deficiency of the thrombin receptor protease-activated receptor 4 (PAR4) abolished the deleterious effects of PN-1 deficiency in hematopoietic cells. These data demonstrate that prevention of thrombin signaling by PN-1 constitutes an important endogenous mechanism of protection against lung fibrosis and associated mortality. Our findings suggest that appropriate doses of thrombin inhibitors or PAR4 antagonists may provide benefit against progressive lung fibrosis with evidence of deregulated thrombin activity.

Dates and versions

hal-03906160 , version 1 (19-12-2022)

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Deborah François, Véronique Arocas, Laurence Venisse, Karen Aymonnier, Leila Idir, et al.. Hematopoietic protease nexin-1 protects against lung injury by preventing thrombin signaling in mice. Blood Advances, 2018, 2 (18), pp.2389-2399. ⟨10.1182/bloodadvances.2018018283⟩. ⟨hal-03906160⟩
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