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Characterization and functional interrogation of the SARS-CoV-2 RNA interactome

Abstract : Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 pandemic, which has led to a devastating global health crisis. The emergence of variants that escape neutralizing responses emphasizes the urgent need to deepen our understanding of SARS-CoV-2 biology. Using a comprehensive identification of RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors that interact with the SARS-CoV-2 genome during infection. By systematically knocking down their expression in human lung epithelial cells, we find that the majority of the identified RBPs are SARS-CoV-2 proviral factors. In particular, we show that HNRNPA2B1, ILF3, QKI, and SFPQ interact with the SARS-CoV-2 genome and promote viral RNA amplification. Our study provides valuable resources for future investigations into the mechanisms of SARS-CoV-2 replication and the identification of host-centered antiviral therapies.
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Contributor : Equipe HAL Université Paris Cité Connect in order to contact the contributor
Submitted on : Monday, June 13, 2022 - 2:08:48 PM
Last modification on : Saturday, September 24, 2022 - 3:10:05 PM


Distributed under a Creative Commons Attribution 4.0 International License

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Athéna Labeau, Luc Fery-Simonian, Alain Lefevre-Utile, Marie Pourcelot, Lucie Bonnet-Madin, et al.. Characterization and functional interrogation of the SARS-CoV-2 RNA interactome. Cell Reports, 2022, 39 (4), pp.110744. ⟨10.1016/j.celrep.2022.110744⟩. ⟨hal-03694154⟩



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