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Bilirubin oxidase-like proteins from P odospora anserina : promising thermostable enzymes for application in transformation of plant biomass

Abstract : Plant biomass degradation by fungi is a critical step for production of biofuels, and laccases are common ligninolytic enzymes envisioned for ligninolysis. Bilirubin oxidases (BODs)-like are related to laccases, but their roles during lignocellulose degradation have not yet been fully investigated. The two BODs of the ascomycete fungus Podospora anserina were characterized by targeted gene deletions. Enzymatic assay revealed that the bod1(Δ) and bod2(Δ) mutants lost partly a thermostable laccase activity. A triple mutant inactivated for bod1, bod2 and mco, a previously investigated multicopper oxidase gene distantly related to laccases, had no thermostable laccase activity. The pattern of fruiting body production in the bod1(Δ) bod2(Δ) double mutant was changed. The bod1(Δ) and bod2(Δ) mutants were reduced in their ability to grow on ligneous and cellulosic materials. Furthermore, bod1(Δ) and bod2(Δ) mutants were defective towards resistance to phenolic substrates and H2 O2 , which may also impact lignocellulose breakdown. Double and triple mutants were more affected than single mutants, evidencing redundancy of function among BODs and mco. Overall, the data show that bod1, bod2 and mco code for non-canonical thermostable laccases that participate in the degradation of lignocellulose. Thanks to their thermal stability, these enzymes may be more promising candidate for biotechnological application than canonical laccases.
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Submitted on : Friday, February 26, 2021 - 2:44:40 PM
Last modification on : Thursday, June 3, 2021 - 3:36:16 AM

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Ning Xie, Gwenaël Ruprich-Robert, Philippe Silar, Florence Chapeland-Leclerc. Bilirubin oxidase-like proteins from P odospora anserina : promising thermostable enzymes for application in transformation of plant biomass. Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2015, 17 (3), pp.866-875. ⟨10.1111/1462-2920.12549⟩. ⟨hal-03153555⟩

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