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Identification of NoxD/Pro41 as the homologue of the p22 phox NADPH oxidase subunit in fungi

Abstract : NADPH oxidases (Nox) are membrane complexes that produce O2(-). Researches in mammals, plants and fungi highlight the involvement of Nox-generated ROS in cell proliferation, differentiation and defense. In mammals, the core enzyme gp91(phox)/Nox2 is associated with p22(phox) forming the flavocytochrome b558 ready for activation by a cytosolic complex. Intriguingly, no homologue of the p22(phox) gene has been found in fungal genomes, questioning how the flavoenzyme forms. Using whole genome sequencing combined with phylogenetic analysis and structural studies, we identify the fungal p22(phox) homologue as being mutated in the Podospora anserina mutant IDC(509). Functional studies show that the fungal p22(phox), PaNoxD, acts along PaNox1, but not PaNox2, a second fungal gp91(phox) homologue. Finally, cytological analysis of functional tagged versions of PaNox1, PaNoxD and PaNoxR shows clear co-localization of PaNoxD and PaNox1 and unravel a dynamic assembly of the complex in the endoplasmic reticulum and in the vacuolar system.
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Submitted on : Friday, February 26, 2021 - 2:38:53 PM
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Isabelle Lacaze, Hervé Lalucque, Ulrike Siegmund, Philippe Silar, Sylvain Brun. Identification of NoxD/Pro41 as the homologue of the p22 phox NADPH oxidase subunit in fungi. Molecular Microbiology, 2015, 95 (6), pp.1006-1024. ⟨10.1111/mmi.12876⟩. ⟨hal-03153545⟩



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