 |
Journal articles
Screen for soil fungi highly resistant to dichloroaniline uncovers mostly Fusarium species
Abstract : Arylamines are frequent pollutants in soils. Fungi have proven to be efficient in detoxifying these chemicals by acetylating them using arylamine N-acetyl transferase enzymes. Here, we selected from natural soils fungi highly resistant to 3,4-dichloroaniline (DCA). Fusarium species were the most frequently isolated species, especially Fusarium solani. The sequenced strain of F. solani contains five NAT genes, as did all the DCA-resistant isolates. RT-PCR analysis showed that the five genes were expressed in F. solani. Expression of the F. solani genes in Podospora anserina and analysis of acetylation directly in F. solani showed that only the NhNAT2B gene conferred significant resistance to DCA and that F. solani likely uses pathways different from acetylation to resist high doses of DCA, as observed previously for Trichoderma.
https://hal-univ-paris.archives-ouvertes.fr/hal-03153538
Contributor : Nadia Berkoun <>
Submitted on : Friday, February 26, 2021 - 2:35:06 PM
Last modification on : Saturday, June 19, 2021 - 4:06:45 AM
Contributor : Nadia Berkoun <>
Submitted on : Friday, February 26, 2021 - 2:35:06 PM
Last modification on : Saturday, June 19, 2021 - 4:06:45 AM