An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures

Marie Bidart (1) , Isabelle Bonnet (1) , Aurélie Hennebique (1) , Zine Eddine Kherraf (1) , Hervé Pelloux (1) , François Berger (1) , Muriel Cornet (1, 2) , Sébastien Bailly (1, 3) , Danièle Maubon (1, 2)

1 CHUGA - Centre Hospitalier Universitaire [CHU Grenoble]
2 TIMC-IMAG-TheREx - Thérapeutique Recombinante Expérimentale
3 IAME (UMR_S_1137 / U1137) - Infection, Anti-microbiens, Modélisation, Evolution

We developed an in-house assay for the direct identification, by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, of yeasts in blood culture. Sixty-one representative strains from 12 species were analyzed in spiked blood cultures. Our assay accurately identified 95 of 107 (88.8%) positive blood cultures and outperformed the commercial Sepsityper kit (81.7% identification).

Domaines: Mycologie

Marie Bidart, Isabelle Bonnet, Aurélie Hennebique, Zine Eddine Kherraf, Hervé Pelloux, et al.. An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures. Journal of Clinical Microbiology, 2015, 53 (5), pp.1761-1764. ⟨10.1128/JCM.03600-14⟩. ⟨hal-03034336⟩