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Journal articles
Transcriptional Silencing of the TFPI-2 Gene by Promoter Hypermethylation in Choriocarcinoma Cells
Abstract : Tissue factor pathway inhibitor-2 (TFPI-2), a Kunitztype serine proteinase inhibitor associated with the extracellular matrix, has been shown to reduce tumor invasion. In the present study we identified the presence of a complete CpG island region spanning exon 1 and the three transcription initiation sites. We demonstrate that DNA demethylation by 5'-aza-2'-deoxycytidine restores TFPI-2 transcription in JAR choriocarcinoma cells. The effect of in vitro DNA methylation on TFPI-2 promoter function was also confirmed with TFPI-2/luciferase promoter constructs. Finally, we determined the precise methylation status of CpG sites of the TFPI-2 promoter in normal and tumor trophoblast cells using the bisulfite genomic sequencing method. We conclude that hypermethylation of the TFPI-2 gene is correlated with transcriptional silencing and that the TFPI-2 gene may be a candidate tumor suppressor gene.
Cited literature [22 references]
https://hal-univ-paris.archives-ouvertes.fr/hal-02127398
Contributor : Florent Hube <>
Submitted on : Monday, May 13, 2019 - 2:05:45 PM
Last modification on : Thursday, October 1, 2020 - 9:33:13 AM
Contributor : Florent Hube <>
Submitted on : Monday, May 13, 2019 - 2:05:45 PM
Last modification on : Thursday, October 1, 2020 - 9:33:13 AM
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04-2003-Biol Chem.pdf
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